Journal: PLoS ONE

Article Title: Methyl Sulfone Blocked Multiple Hypoxia- and Non-Hypoxia-Induced Metastatic Targets in Breast Cancer Cells and Melanoma Cells

doi: 10.1371/journal.pone.0141565

Figure Lengend Snippet: (A) 66cl-4 metastatic breast cancer cells were grown to confluence in the presence and absence of methyl sulfone. At confluence, half of the control plates (no methyl sulfone) and half of the methyl sulfone-treated plates were placed under hypoxia at 37°C. The remaining plates continued incubation under normoxic conditions. After six hours cells were solubilized in TRIzol to isolate RNA. Relative levels of miRNA-210-3p and miR-210-5p were determined by microarray analysis and real time QPCR, in triplicate. Six pairwise comparisons of the four samples for each miR are shown (A) along with Log2 differences between pairs. (B) Relative levels of individual miR-210 were determined.

Article Snippet: LC Sciences performed RNA quality tests and then analyzed RNA samples with real time QPCR for relative levels of miR-210-3p and miR-210-5p using their MicroRNA Service.

Techniques: Control, Incubation, Microarray

Journal: BMC Evolutionary Biology

Article Title: Comparative analyses of glycerotoxin expression unveil a novel structural organization of the bloodworm venom system

doi: 10.1186/s12862-017-0904-4

Figure Lengend Snippet: Quantitative real-time PCR (qPCR) expression levels (shown as Fold Change, RQ) between biological groups (putative venom glands (pvg), pharyngeal lobes, and posterior body wall) of five analyzed GLTx transcripts (GLTx paralog 1–3, and adjacent gene regions GLTx-3’ and GLTx-5’; for details see Material and methods section “Quantitative real-time PCR”) in G. tridactyla ( n = 10; *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05). a Relative GLTx expression (logarithmic scale) in putative venom glands (grey) and pharyngeal lobes (orange) in comparison to the GLTx expression signal exhibited by the body tissue (RQ = 1). Relative GLTx expression in the pharyngeal lobes and putative venom glands is significantly different from the expression signal in the body tissue. b Relative GLTx expression (linear scale) within the pharyngeal lobes in comparison to the putative venom glands (RQ = 1). Relative GLTx expression is significantly different between both putative venom glands and pharyngeal lobes

Article Snippet: Quantitative real-time PCR (qPCR) experiments were performed on Glycera tridactyla Schmarda, 1861 (Annelida, Glyceridae) specimens obtained from the Roscoff marine biological station in February 2015.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Comparison

Journal: Best Practice & Research. Clinical Rheumatology

Article Title: Laboratory evaluation of SARS-CoV-2 in the COVID-19 pandemic

doi: 10.1016/j.berh.2021.101660

Figure Lengend Snippet: EUA assay sensitivity and instructions for SARS-CoV-2 molecular assays.

Article Snippet: ScienCell Research Laboratories , ScienCell SARS-CoV-2 Coronavirus Real-time RT-PCR (RT-qPCR) Detection Kit , Kit , 3-Apr-20 , X , , , , , X , X , X , X , .

Techniques: Control, Quantitative RT-PCR, Diagnostic Assay, Reverse Transcription, Luminex, Detection Assay, Reverse Transcription Polymerase Chain Reaction, CRISPR, Real-time Polymerase Chain Reaction, Sequencing, Multiplex Assay

Journal: Best Practice & Research. Clinical Rheumatology

Article Title: Laboratory evaluation of SARS-CoV-2 in the COVID-19 pandemic

doi: 10.1016/j.berh.2021.101660

Figure Lengend Snippet: EUA assay targets and specimen types for SARS-CoV-2 molecular assays.

Article Snippet: ScienCell Research Laboratories , ScienCell SARS-CoV-2 Coronavirus Real-time RT-PCR (RT-qPCR) Detection Kit , Kit , 3-Apr-20 , X , , , , , X , X , X , X , .

Techniques: Control, Quantitative RT-PCR, Diagnostic Assay, Reverse Transcription, Luminex, Detection Assay, Reverse Transcription Polymerase Chain Reaction, CRISPR, Real-time Polymerase Chain Reaction, Sequencing, Multiplex Assay